Antioxidants inhibit mercuric chloride-induced early vasculitis.
نویسندگان
چکیده
In the Brown Norway (BN) rat, mercuric chloride (HgCl(2)) induces a T(h)2-dominated autoimmune syndrome which includes an early phase of mast cell-dependent vasculitis. We have shown in vitro that oxidative stress up-regulates IL-4 in mast cells and predisposes to degranulation. The aim of this study was to determine whether administration of antioxidants inhibits HgCl(2)-induced early vasculitis in vivo, and, if so, to examine whether modulation of the oxidative/antioxidative balance influences IgE and IL-4 expression by mast cells in situ. Groups of rats were given HgCl(2) + saline, HgCl(2) + N-acetyl-L-cysteine (NAC), saline + saline or saline + NAC respectively and blood was taken and animals killed 48 h later. NAC significantly reduced both HgCl2-induced early vasculitis and HgCl(2)-enhanced IgE expression on mast cells with a trend to a decrease in HgCl(2)-enhanced IL-4 expression in these cells. In addition, there was an increased rat mast cell protease (RMCP) II concentration in the serum after HgCl(2) injection and the elevated levels of RMCP II stimulated by HgCl(2) were totally abolished by the administration NAC in the HgCl(2) + NAC group. However, there was no significant change in serum total IgE concentrations between the HgCl(2) + saline group and the HgCl(2) + NAC group. The non-sulphydryl-containing antioxidants desferrioxamine and pyruvate demonstrated a similar effect in inhibiting HgCl(2)-induced early vasculitis. Our data show that administration of an antioxidant to BN rats reduces HgCl(2)-induced early vasculitis, suggesting that oxidative stress plays a role in the pathogenesis of HgCl(2)-induced early vasculitis. This finding may have implications for the understanding of the initiation in this experimental model of T(h)2 cell-driven autoimmunity and possibly of analogous human diseases.
منابع مشابه
Modulating Role of Panax Ginseng in Phase - II Reaction of Hepato - Biotransformation in Albino Rats Following Mercuric Chloride Intoxication
Introduction: The fate of xenobiotics that is present, increasing day by day. The increasing fates altered or inhibit the metabolic activities like detoxification and biotransformation. Methods: The present study highlights this slow biotransformation and detoxification on the basis of specific enzymes which have a say in assessment of mercuric chloride toxicity and modulation by Panax ginse...
متن کاملMercuric Chloride-Induced Vasculitis in the Brown Norway Rat: a p T Cell-Dependent and -Independent Phases
متن کامل
Spirulina fusiformis provides protection against mercuric chloride induced oxidative stress in Swiss albino mice.
Oxidative stress induced by mercuric chloride (5 mg/kg body weight i.p.) in mice substantially increases the lipid peroxidation level along with corresponding decrease in the reduced glutathione and various antioxidant enzymes in liver and increase in serum transaminases activity. Supplementation of Spirulina (800 mg/kg body weight orally, in olive oil, along with mercuric chloride) for 40 days...
متن کاملMercuric Chloride Induced Cell Death in Spinal Cord of Embryo in Rat
A B S T R A C TIntroduction: Because of more exposure to mercury compounds, the prenatal and postnatal neurotoxic effects of mercury compounds have gained more attention in last decade. The aim of this study was to investigate the effects of mercuric chloride intoxication on spinal cord development during prenatal period. Methods: 36 adult Sprague-dawley rats after observing vaginal mating plaq...
متن کاملEffect of Sodium Selenide on Renal Toxicity Induced By Mercuric Chloride in Rat
Background and Aims: Mercury, with its oxidative activity, causes damage to the antioxidant enzymes thus resulting in physiological disorders. Sodium selenide is an antioxidant that protects antioxidant enzymes. The aim of this study was to investigate the protective effect of sodium selenide on renal toxicity induced by mercuric chloride in rats. Materials and Methods: Animals were divided in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International immunology
دوره 14 3 شماره
صفحات -
تاریخ انتشار 2002